Previous studies have reported that GPC3 alone cannot distinguish HCC from DN, and a combination of GPC3, HSP70, and GS has been used for the diagnosis of HCC, where the positive expression of more than two of these three markers suggested a diagnosis of HCC.26 Our results demonstrated that AFP mRNA has a specificity of close to 100% in both surgical and biopsy samples. This evidence concerns the gene GPC3 and hepatocellular carcinoma.