Factors that were significant on univariate analysis as well as those with P values < 0.20 (male sex, ASA score ≥ 2, diabetes mellitus with insulin use, use of a bio-clean room, prophylactic intravenous administration of cefazolin, and operative time) were subjected to multivariable analysis for further examination of the risk variables for deep SSI. This evidence concerns the gene INS and diabetes mellitus.