To examine the potential of inducing uracil DNA misincorporation as an effective therapeutic strategy to enhance TNBC response to TS inhibitors and anthracyclines, the catalytic activity of dUTPase was blocked, either by small interfering RNA (siRNA) or by small-molecule inhibition and the impact on cancer cell proliferation and survival in combination with anthracyclines or TS inhibitors was evaluated. Here, TYMS is linked to cancer.