Kaplan–Meier survival analysis further indicated that patients with HCC, with low expression levels of SLC25A20, had a significant shorter overall survival (OS) and recurrence-free survival (RFS) compared with those patients with high SLC25A20 expression levels (Fig. 1F), which was validated by bioinformatic analysis based on the online web portal Kaplan–Meier Plotter12 (Fig. 1G). This evidence concerns the gene SLC25A20 and hepatocellular carcinoma.