Besides, we found that miR-16 family/TFAP2A/PSG9 had no relationship with tumor size (Fig. 7E), but was obviously related to lymph node metastasis, that is the miR-16 family- TFAP2A+PSG9+ had the highest lymph node metastasis rate, and the miR-16 family + TFAP2A- PSG9- had the lowest lymph node metastasis rate (except for miR-424 signal, whose lowest rate was Other group), while the miR-16 and miR-497 signals possessed statistical significance, and p-values of miR-195 and miR-424 were 0.0907 and 0.1165 (Fig. 7F). This evidence concerns the gene PSG9 and neoplasm.