To assess the functional consequences of the L16R mutation, p16-null Panc1 and MiaPaca2 pancreatic cancer cells (21), and an hTERT-immortalized human pancreatic epithelial nestin-expressing cell line that expresses KRASG12V and an shRNA against p16 (22) (referred to HPNE cells hereafter) were transiently transfected with FLAG-tagged p16-WT or p16-L16R. This evidence concerns the gene NES and familial pancreatic carcinoma.