After GSPT1 was silenced, the expression of GSK-3β, p21, and p27 increased, and the expression of CyclinD1, CDK4/6, cyclinE, and CDK2 decreased; these changes affected the progression of colon cancer cells from G1 phase to S phase and blocked the cell cycle. The gene discussed is GSK3B; the disease is malignant colon neoplasm.