We used published stromal singlecell RNA sequencing data from DSS colitis models,10 in combination with dualISH/IHC, to assess the coexpression and spatial distribution of established andfunctionally relevant stromal subpopulations within these broader mesenchymalgroups (Figure 4B, Supplementary Figure 5).We were able to find overlap of Grem1 expression with some keymorphogens and cytokines implicated in colonic regeneration, with stromalsubsets coexpressing Grem1 andRspo3,26Ptgs2 (cyclooxygenase-2),27 andIl33. This evidence concerns the gene PTGS2 and colitis.