In the present study, we found that some genes of benefit to nervous system disease were activated (such as Lhx8, GPR88, RGS9, CD4, DRD2, RXRG, and Syt6), following the increase in the number of cholinergic and GABAergic neurons in the HSHF-diet offspring (Figure 4). Here, LHX8 is linked to nervous system disorder.