Afterwards, our research results verified that, the abnormal activation of NUAK1 in resistant CRC cells suppressed PP1β activity, and regulated the phosphorylation level of GSK3βS9 site to induce the nuclear import and transcription activity of Nrf2, up-regulate the levels of intracellular series anti-oxidative molecules, maintain the redox balance in cancer cells, and induce cancer cell resistance. The gene discussed is NUAK1; the disease is cancer.