CD4 and neoplasm: In addition, the positive signals of CD4+ T cells, macrophages (CD68+) and M2 macrophages (CD163+) were located in the microenvironment of the tumor invasion front, while CD8+ T cells were distributed not only in the microenvironment of the tumor invasion front but also within the tumor glands (Fig. 1e–h, Supplementary Table 5).