Meanwhile, the autophagy was restrained with GFRA1 depletion in osteosarcoma cells, and the co-transfection of anti-miR-381-3p and si-GFRA1 recovered the autophagy function of U2OS/DXR and MG63/DXR cells (Figures 7j, k and A1k, l). Here, GFRA1 is linked to osteosarcoma.