BECN1 and osteosarcoma: The expression of LC3-II/LC3-I and Beclin-1 was also elevated, while p62 was downregulated in U2OS/DXR and MG63/DXR cells compared with that in their parental cells (Figures 1e and A1a), suggesting that the DXR resistance of osteosarcoma cells might be related to the autophagy.