The levels of circSAMD4A, miR-218-5p and KLF8 were detected, and the results showed that relative to the non-tumor tissues and normal cell line hFOB, circSAMD4A (Figure 1a and b) and KLF8 (Figure 1i, j, m and n) were elevated, while miR-218-5p (Figure 1e and f) was decreased in osteosarcoma tissues and cell lines (HOS and U2OS). The gene discussed is KLF8; the disease is neoplasm.