In addition, miR-216a expression was negatively regulated by LINC00461 in glioma tissues and cell lines U251 and A172 cells, and downregulation of miR-216a could abolish the suppressive role of LINC00461 knockdown in cell proliferation, migration, invasion, and apoptosis inhibition in vitro. Here, MIR9-2HG is linked to central nervous system cancer.