In addition, miR-216a expression was negatively regulated by LINC00461 in glioma tissues and cell lines U251 and A172 cells, and downregulation of miR-216a could abolish the suppressive role of LINC00461 knockdown in cell proliferation, migration, invasion, and apoptosis inhibition in vitro. The gene discussed is MIR9-2HG; the disease is glioma.