Moreover, the qRT-PCR analysis demonstrated that MYL6B was also upregulated in human rectal adenocarcinoma cell lines (HT-29, SW1116, HCT116, and SW480) at different degrees when compared with that of human normal cell line Hs680.Rec (Figure 1e, P < 0.01). The gene discussed is MYL6B; the disease is rectum adenocarcinoma.