In this study, the glycolysis stress test of ECAR was used to evaluate the effect of LINC00202 on RB cell aerobic glycolysis, and we found that LINC00202 deletion downregulated the level of glycolysis, glycolytic capacity, and glycolytic reserve in RB cells, and the expression of glycolytic enzyme LDHA was also reduced by LINC00202 silence. This evidence concerns the gene LDHA and retinoblastoma.