After that, miR-489-3p expression in GBM was detected, results indicate that the level of miR-489-3p was significantly decreased in GBM tissues (P < 0.0001) and cells (P = 0.0143, P = 0.0029) compared with the NC group (Figure 3d and e), and BDNF expression was negatively correlated with miR-489-3p in GBM tissue (r = −0.9019; P < 0.0001) (Figure 3f). This evidence concerns the gene BDNF and glioblastoma.