Furthermore, the differences in the expression of E-cadherin, Snail and Twist between gastric cancer tissues and lymph node metastasis were not statistically significant (the X2 values were 1.804, 1.541 and 0.419, respectively; and the P values were 0.179, 0.214 and 0.517, respectively). This evidence concerns the gene CDH1 and metastatic malignant neoplasm in the lymph nodes.