To investigate the dynamics of tumor cells resulting from ligand-dependent activation of EPHB4, EPHB4-positive Rh30 cells were co-cultured with human recombinant EPHRIN B2, and the activation of downstream signaling of EPHB4, Akt, and Erk1/2, which are associated with the cellular proliferation machinery of RMS, was evaluated. The gene discussed is AKT1; the disease is neoplasm.