EPHB4-CAR-T or CD19-CAR-T cells were co-cultured with the EPHB4-positive RMS cell line (Rh30) at various effector-to-target (E:T) ratios, such as 2:1, 1:1, or 1:2, and the growth of tumor cells was monitored for over 150 h. The gene discussed is CD19; the disease is neoplasm.