The protein expression of IFITM3 in the QGY-7701, HepG2, and PLC/PRF/5 human HCC cell lines was analyzed by western blotting analysis, and the expression level of IFITM3 protein was higher in PLC/PRF/5 cell line, so the PLC/PRF/5 cell was selected as the optimal experimental cell (Figure 2(a)). The gene discussed is IFITM3; the disease is hepatocellular carcinoma.