Cytoplasmic aggregation of TDP-43 has been observed by overexpressing wild-type and mutant TDP-43 in cellular and animal models of ALS (Barmada et al., 2010; Li et al., 2010; Walker et al., 2015; De Giorgio et al., 2019; Watanabe et al., 2020). Here, TARDBP is linked to amyotrophic lateral sclerosis.