Despite this conservative IFNγ expression, it is highly effective with large increases in IFNγ-dependent signaling including STAT1. Corresponding to increased IFNγ-dependent signaling, STAT1 expression is increased 2-fold in the brain at 14dpi and continues to increase as infection progresses through the mid-chronic stage, levelling off between 28 and 56dpi (Figure 3D, second from left). Here, IFNG is linked to infection.