α4β7+ CD4+ T lymphocyte subset thus plays a crucial role in mucosal transmission (Cicala et al., 2009; Sivro et al., 2018) and we report for the first time that CD4+ T cells expressing both β7 and CCR5 were conducive to trans-infection following co-culture with HIV-1 exposed vaginal epithelial cells. This evidence concerns the gene CD4 and infection.