The number of ZO-1 positive cells (YC vs. YM: P<0.05; OC vs. OM: P<0.01; Figure 7A) and OCLN positive cells (YC vs. YM: P<0.01; OC vs. OM: P<0.01; Figure 7B) in the gut was significantly decreased in the YM and OM groups than those in the age matched controls. The gene discussed is TJP1; the disease is ocular melanoma.