RAF1 and infection: In a fourth pull-down study (Poyomtip et al., 2016), a full-length DENV-2 construct (strain 16681) with tandem affinity purification (TAP)-tagged NS5 containing a poly-histidine and FLAG tags (inserted following N173 in MTase domain of NS5) was propagated in BHK21 cells followed by infection in Huh-7 cells.