In pre-clinical models the most potent abscopal effects have been observed when CTLA4-anatagonist treatment was applied during RT with 3X8Gy (vs. 5X6 Gy) in breast and colon cancer-bearing mice and not with a single dose of 20 or 30 Gy (42, 43). The gene discussed is CTLA4; the disease is malignant colon neoplasm.