In univariate logistic regression analysis, factors with possible impact to nodal disease were incorporated into the model, including clinical pathological parameters such as gender, age, location, tumor size, histological type, differentiation, EGFR, CD34, Ki67, mutant p53, MLH1,MSH2, KRAS, along with TMEM16A mRNA expression and protein expression. The gene discussed is MSH2; the disease is neoplasm.