Recently, growing evidences have shown that TMEM16A is overexpressed or amplified in many tumors, such as head and neck squamous cancer cells (HNSCC), esophageal squamous cell cancer (ESCC), and breast cancer (11–17), and overexpression of TMEM16A plays an important role in the development and progression of tumors (18–22). Here, ANO1 is linked to head and neck squamous cell carcinoma.