In line with these findings, Western blot analysis demonstrated the upregulation of TMPRSS4 in seven pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, CFPAC-1, Hs766t, PANC-1 and SW1990) and in the PDAC tissue in contrast to the primary normal human pancreatic duct epithelial cell (HPDE6-C7) or adjacent non-tumor tissue (Figures 1I,J). Here, TMPRSS4 is linked to familial pancreatic carcinoma.