However, for further validation a biochemical verification was needed; therefore, we performed quantitative PCR with samples from two normal brain tissues obtained from epilepsy surgery (Numbered 1 and 2), three WNT/SHH MBv (Numbered 3–5), and three Group 3 MB (Numbered 6–8) to test the gene expression levels of TSHZ1, MFAP2, GRM8, CCND2, and AP1S2. The gene discussed is TSHZ1; the disease is epilepsy.