MGMT and glioblastoma: Initial qRT-PCR profiling confirmed that the human GBM cell lines LN229 and T98G show a broad-range upregulation of diverse DDR regulators as compared to normal human astrocytes suggesting that DDR activity is indeed increased—irrespective of the O6-methylguanine-DNA-methyltransferase (MGMT) status (Figure 1A and Supplementary Figure 1A) (53).