PRF1 and neoplasm: Next, we used the conventional SPTCL markers to examine the separation of normal and malignant cells above. As expected, we found that the classical SPTCL marker MKI67 was very specifically observed in malignant cells but mostly not seen in normal cells, while the markers GZMB and PRF1 were not only expressed in the tumor T cells but also in part of normal CD8+ T cells (Supplementary Figure S7).