To further investigate the effect of HOTAIRM1 down-regulation in t-FB1 and t-FB2 cells on tumorigenicity ability of GSCs, in vivo combined inoculation of GSCs-SU3 and HOTAIRM1 downregulated t-FB1/2 cells was performed, the transplanted tumor volume and weight were significantly decreased, compared with the control group (Supplementary Figures 2A–F). The gene discussed is HOTAIRM1; the disease is neoplasm.