The infiltrations of T cells CD8 and T cells follicular helper were significantly down-regulated in GBM outside the VLSM-determined area compared with those in GBM in the VLSM-determined area (T cells CD8, P = 1.00E-02; T cells follicular helper, P = 1.00E-02; Student's t-test, Supplementary Table 4). The gene discussed is CD8A; the disease is glioblastoma.