ANK1 and Alzheimer disease: Similarly, 5mC and 5hmC levels at the two methylation sites in ANK1 that are known to be hypermethylated in AD (chr8:41519308 – cg05066959 and chr8:41519399 – cg11823178) were not correlated with H3K27me3 levels across chr8:41519216-41519359 (primer Set 2) and chr8:41519342-41519460 (primer Set 3), respectively.