To assess the histone modification profile of the ANK1 gene in AD and to see if there was any correlation with the previously reported DNA hypermethylation at this locus, this study utilized ChIP quantitative PCR (ChIP-qPCR) to quantify H3K4me3 and H3K27me3 modifications in the ANK1 gene in post-mortem brain samples from donors with varying degrees of AD pathology. The gene discussed is ANK1; the disease is Alzheimer disease.