However, ischemia-induced tissue inflammation and angiogenesis are commonly observed in both male and female mice after femoral artery ligation, and since PD-1 regulates inflammation in mice of both genders in various disease models, we anticipate that PD-1 inhibition would have similar impacts on tissue inflammation and angiogenesis after hindlimb ischemia in both sexes. Here, PDCD1 is linked to ischemia.