MAX and neoplasm: Subsequently, given the unexpected association of neuroblastoma with a variant of the MAX gene, the presence of loss of heterozygosity (LOH) was evaluated in a formalin–fixed and paraffin–embedded sample of the neuroblastoma tumour (from a delimited area with >50% tumour cells), using the same NGS panel, and the variant allele frequency (VAF) was 71.2%, which is compatible with LOH.