In response to a HFD with a macronutrient composition and cholesterol content that is comparable to human diets (23–25), Ldlr-/-.Leiden mice develop histopathological characteristics of human NAFLD in the context of an obese phenotype with dyslipidaemia, hypertriglyceridaemia and insulin resistance (26–28) as is typical for a large proportion of NAFLD patients (29–31). Here, LDLR is linked to metabolic dysfunction-associated steatotic liver disease.