PHOX2B and neuroblastoma: In particular, in vitro transfections of the two c.*161 allele reporter constructs did not reveal any effect of miR-204 on the G allele, while the presence of the A allele was always associated with a marked decrease in Luciferase's activity, likely reflecting its role on the entire 3′UTR PHOX2B. To perform such studies, human neuroblastoma IMR32 cells were used as expressing high PHOX2B levels and already demonstrated suitable to study PHOX2B post-transcriptional regulation (26).