CRH and major depressive disorder: In line with previous reports (Fuentes and Christianson, 2018; Tang et al., 2017), we confirmed that MS Vgat-Cre mice presented pronounced visceral hypersensitivity and anxiety- and depression-like behaviors in adulthood, coupled with upregulation of CRF protein expression and activation of CRF neurons in PVN (Figures 1, 2).