ADAM8 and metabolic dysfunction-associated steatotic liver disease: To further study whether the expression levels of ADAM8 can be regulated under conditions of NAFLD in vitro, HepG2 and Hepa1-6 cells were treated with fatty acids (a mixture of oleic acid and linoleic acid) and interleukin-1β (IL-1β) alone or in combination to mimic the fatty liver condition [18].