The number of NeuN-positive cells in the cortex of CKD mice treated with metformin was significantly higher than that in vehicle-treated animals (CKD-met: 87.92 ± 7.51 vs. CKD-veh: 57.76 ± 13.71% of NeuN-positive cells, p < 0.001) (Fig. 2B). This evidence concerns the gene RBFOX3 and chronic kidney disease.