Expression levels of Shh signaling pathway genes (IHH, BOC, and RAB23) and their regulatory miRNAs (miR-195-5p, miR-6738-3p, and miR-509-3-5p) were evaluated in 50 gastric cancer patients using comparative relative real time PCR, and by comparing the expression in tumor tissues with their paired normal counterpart tissues. This evidence concerns the gene IHH and gastric cancer.