Expression levels of Shh signaling pathway genes (IHH, BOC, and RAB23) and their regulatory miRNAs (miR-195-5p, miR-6738-3p, and miR-509-3-5p) were evaluated in 50 gastric cancer patients using comparative relative real time PCR, and by comparing the expression in tumor tissues with their paired normal counterpart tissues. The gene discussed is SHH; the disease is neoplasm.