Moreover, association study between Shh signaling pathway genes and their regulatory miRNAs according to age groups indicated that IHH and miR-6738-3p expression was significantly (P < 0.05) decreased in gastric cancer patients aged less than 65 years while miR-195-5p was significantly P < 0.05) down-regulated in gastric cancer patients older than 65 years. This evidence concerns the gene IHH and gastric cancer.