In our study, ROS biosynthesis was examined only in alveolar macrophages analyzed after ex vivo culture for 18 h, while the estimation of lipid content and the immunofluorescencent visualization of iNOS and COX-2 expression in the cells were performed both on the histological sections and in the ex vivo cell cultures obtained from the same lung lesions for each TB patient (Figure 4A–E). The gene discussed is NOS2; the disease is tuberculosis.