To determine the pattern of clock related genes more specifically in esophageal SCC epithelial cells, we have used RNA sequencing to profile a commercially available non-immortalized human esophagus epithelial cell line as well as 5 different esophageal SCC cell lines from the KYSE series: KYSE-140, −180, −270, −10 and −450 [20] (Figure 2A). The gene discussed is CLOCK; the disease is esophageal squamous cell carcinoma.