We used two approaches; either using the concentration of the individual TP53 transcripts (i.e., copies of each transcript/μg tumor RNA) or the ratio of each of the individual TP53 transcripts to the t1 transcript (encoding FL/∆40p53α), as this was the most abundant transcript, and since the use of relative transcript levels provides an internal control for sample-specific factors such as differences in efficiency of cDNA synthesis between tumor samples. The gene discussed is TP53; the disease is neoplasm.