Briefly, A375 BRAFV600E human melanoma cells with stable firefly luciferase reporter genes driven by a hybrid EF1α/eIF4g promoter (named A375IF4g/Luc) were injected into the tail vein of NOD/SCID mice, and the lung metastases lesions were primarily cultured ex vivo to recover the metastatic A375LMIF4g/Luc cells. Here, EIF4G1 is linked to melanoma.