A transcriptome analysis of 15 human lung cancer samples and 14 colorectal cancer samples demonstrated that tumor-infiltrating Treg cells have very high levels of different Treg activation markers, such as T cell immunoglobulin mucin receptor 3 (HAVCR2), glucocorticoid-induced TNFR-related protein (GIRT), lymphocyte-activation gene 3 protein (LAG3), and inducible T cell co-stimulator (ICOS). This evidence concerns the gene ICOS and neoplasm.