Because CtIP and CCAR2 were previously identified as mechanistic targets for SFN and SFN + JQ1, respectively, we used CRISPR/Cas9 in HCT116 colon cancer cells and observed markedly higher basal ERCC2 expression in CtIP–/– and CCAR2–/– cell lines compared to the parental cell line (Figure S5). Here, ERCC2 is linked to colonic neoplasm.