TGFB1 and metastatic malignant neoplasm in the lymph nodes: For the TGFβ and FOXO pathways, activity scores were generally lower in lymph node metastases compared to the PT, with lower FOXO indicating higher PI3K pathway activity; for AR, HH and WNT pathways, activity scores were more frequently higher in lymph node metastases compared to the PT (Figure 5A, Supplementary Materials Statistics, Tables S8–S10).