Furthermore, concentrations of 4-HNE protein adducts, shown to be also triggered in liver tissue through TLR4-dependent signaling pathways in settings of NAFLD [35], were significantly higher in the liver tissue of FFC-fed mice than in C- and FFC + S–fed mice (p ≤ 0.05) (Figure 3 and Figure S1). Here, TLR4 is linked to metabolic dysfunction-associated steatotic liver disease.